Journal: bioRxiv

Article Title: Nanoliposomal Omega-3 Fatty Acids Promote Adult Hippocampal Neurogenesis through the BDNF/TrkB Pathway in C57BL/6 Mice

doi: 10.64898/2026.02.24.707750

Figure Lengend Snippet: Protein expression of molecules from the BDNF signaling pathway determined by Western Blot and representative membranes. (A)mBDNF. (B)TrkB. (C)proBDNF. (D)p75 for yogurt treated mice after 4 weeks. (E–F) Representative membranes showing signals for TrkB, mBDNF, pro-BDNF, and p75 in Control and fatty acids-treated mice. Data are expressed as mean ± SD, n =5−6/group. # 0.05 < p < 0.10; *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Samples (50 μgr in 5× loading buffer) were then loaded into SDS-PAGE gels (12 or 15%) and transferred onto nitrocellulose membranes using the Mini-PROTEAN ® Tetra System (BIO-RAD) for 1 h. Membranes were incubated for 1 h with blocking solution (5% milk in TBST) and then probed overnight at 4°C with mouse anti-BDNF (1:2000; Icosagen; 327-100 clone 3C11), rabbit anti-p75 (1:700; Alomone Labs; ANT-007), rabbit anti-TrkB (1:700; Alomone Labs; ANT-019), and rabbit anti-proBDNF (1:2500; Genocopoeia) in TBST.

Techniques: Expressing, Western Blot, Control

Journal: bioRxiv

Article Title: GM1 and GD3 Gangliosides Attenuate NGF-TrkA and BDNF-TrkB Signaling Dysfunction Associated with Acute Diisopropylfluorophosphate Exposure in Mouse Brain

doi: 10.1101/2025.03.31.646417

Figure Lengend Snippet: Mature BDNF and proBDNF levels were measured by Western blot in brain lysates derived from mice exposed to either vehicle or DFP (4.0 mg/kg), followed by treatment with saline, GM1, or GD3 (5 mg/kg/day for 7 days). (A) Representative blot illustrating proBDNF (32 kDa) and BDNF (14 kDa). CTX, cortex; PFC, prefrontal cortex; HIP, hippocampus; SVZ, subventricular zone. (B) The data presented in the bar graphs were obtained from densitometry measurements of the bands for proBDNF, BDNF, and actin . Ratios of proBDNF and BDNF to actin were calculated for each replicate sample. Results are expressed as means ± S.E.M. of ≥3 replicates per condition. *P < 0.05 and **P < 0.01 indicates significant differences between indicated groups, as calculated using one-way ANOVA with Tukey’s multiple comparison test.

Article Snippet: For this study, the following antibodies were purchased: rabbit anti-TrkA (RRID:AB_310180; Millipore Millipore, St. Louis, MO, USA, #06-574), mouse anti-NeuN (RRID:AB_2298772; Sigma-Aldrich, St. Louis, MO, USA, #MAB377), rabbit anti-p75 neurotrophin receptor (p75 NTR , RRID:AB_310649; Millipore #07-476), rabbit anti-sortilin (RRID:AB_2192606; Abcam, Cambridge, MA, USA, #ab16640), goat anti-TrkB (RRID:AB_2155263; R&D Systems, Minneapolis, MN, USA, #BAF1494), rabbit anti-actin (RRID:AB_476693; Sigma #A2066), rabbit anti-NGF (RRID:AB_2040019, Alomone Labs, Jerusalem, Israel, #AN-240), rabbit anti-brain-derived neurotrophic factor (BDNF) (RRID:AB_2039756, Alomone Labs #ANT-010), and rabbit anti-phospho-Trk (RRID:AB_2298805; Cell Signaling Technology, Danvers, MA, USA, #9141).

Techniques: Western Blot, Derivative Assay, Saline, Comparison